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- Nplate (Romiplostim) Neutralizing Antibody, Mouse Monoclonal (Clone 2A3-B6) [MA202N-100 or MA202N-025]
Nplate (Romiplostim) Neutralizing Antibody, Mouse Monoclonal (Clone 2A3-B6) [MA202N-100 or MA202N-025]
Product Description
Nplate (Romiplostim) Neutralizing Antibody, Mouse Monoclonal (Clone 2A3-B6) [MA202N-100 or MA202N-025]
Nplate (Romiplostim) is a thrombopoietin receptor (TPO-R) agonist marketed by Amgen for the treatment of thrombocytopenia (Reference 1, see below). It has been approved for the treatment of both adult and pediatric patients with immune thrombocytopenia (ITP), a condition that occurs when the blood platelet count is too low. Nplate (Romiplostim) is a recombinant human IgG Fc fusion protein produced in E. coli (2).
GenHunter has 2 neutralizing monoclonal antibodies against Nplate (Romiplostim): Clone 5D5-G2 and Clone 2A3-B6. These NAbs have specificity against TPO-R Mimetic Peptide (Nplate).
Nplate (Romiplostim) Neutralizing Antibody Determination: Nplate neutralizing antibody assay for the two mAbs was carried out with Ba/F3 (c-Mpl) cell bioassay method in which Ba/F3 (c-Mpl) cells were grown in the presence of Nplate with or without the mAbs. EC50 (see data in specifications tab below) was determined at the concentration where a mAb caused 50% inhibition of the cell proliferation.
Nplate (Romiplostim) Antibody Products Available:
Monoclonal Antibody Description | Clone | Isotype | Specificity | Volume | Applications |
Cat. No. |
Click to Order |
Nplate (Romiplostim) Neutralizing Antibody, Mouse Monoclonal (Clone 5D5-G2) | 5D5-G2 | IgG1,Kappa |
TPO-R Mimetic |
100µg or 25µg |
NAb,ELISA,WB |
Order | |
Nplate (Romiplostim) Neutralizing Antibody, Mouse Monoclonal (Clone 2A3-B6) | 2A3-B6 | IgG1,Kappa |
TPO-R Mimetic |
100µg or 25µg |
NAb,ELISA,WB |
Order |
We also have many other antibody and antigen products (CLICK HERE or names below) including for:
SARS-CoV-2 (COVID-19), Human IL-24, Human IL-20R2, Nplate (Romiplostim), Human VEGF-R, Endo180 (uPARAP, CD280), Tumor Necrosis Factor (TNF) superfamily, SIRPα (Signal-regulatory protein alpha), Rabies virus, Trimer-Tag, RSV (Respiratory Syncytial Virus), HSV-2 (Herpes Simplex Virus 2), and AP (alkaline phosphatase).
References:
- Kuter, D.J. et al: Efficacy of romiplostim in patients with chronic immune thrombocytopenic purpura: a double-blind randomised controlled trial. (2008) The Lancet. 371:395-403. https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(08)60203-2/abstract
- Cines, D.B. et al: Romiplostim. (2008) Nature Reviews Drug Discovery. 7:887-888. https://www.nature.com/articles/nrd2741
All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Antibody Specifications | |
Antibody Name |
Nplate (Romiplostim) Neutralizing Antibody, Mouse Monoclonal (Clone 2A3-B6) |
Clone No. | 2A3-B6 |
Class | Mouse Monoclonal |
Catalog No / SKU | MA202N-100 (for 100 µg) or MA202N-025 (for 25 µg) |
IgG subtype | IgG1, Kappa |
Specificity | TPO-R Mimetic Peptide (Nplate) |
Binding / Neutralizing mAb | Neutralizing mAb (NAb) |
Volume | 25 or 100 ug |
Provided as | Liquid |
Applications | Neutralizing antibody: EC50 = 9.2 ng/mL to Nplate. ELISA: antigen binding affinity EC50 = 45 ng/mL to Nplate. Western Blot at 1:1800 - 1:3600 |
Concentration | ~ 1 mg/mL |
Purification | G protein affinity purified, >99% determined by HPLC |
Storage conditions | Store at -20°C long term, Avoid freeze / thaw cycle |
All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Documents
Nplate (Romiplostim) Antibody Products Available:
Monoclonal Antibody Description | Clone | Isotype | Specificity | Volume | Applications |
Cat. No. |
Click to Order |
Nplate (Romiplostim) Neutralizing Antibody, Mouse Monoclonal (Clone 5D5-G2) | 5D5-G2 | IgG1,Kappa |
TPO-R Mimetic |
100µg or 25µg |
NAb,ELISA,WB |
Order | |
Nplate (Romiplostim) Neutralizing Antibody, Mouse Monoclonal (Clone 2A3-B6) | 2A3-B6 | IgG1,Kappa |
TPO-R Mimetic |
100µg or 25µg |
NAb,ELISA,WB |
Order |
We also have many other antibody and antigen products (CLICK HERE or names below) including for:
SARS-CoV-2 (COVID-19), Human IL-24, Human IL-20R2, Nplate (Romiplostim), Human VEGF-R, Endo180 (uPARAP, CD280), Tumor Necrosis Factor (TNF) superfamily, SIRPα (Signal-regulatory protein alpha), Rabies virus, Trimer-Tag, RSV (Respiratory Syncytial Virus), HSV-2 (Herpes Simplex Virus 2), and AP (alkaline phosphatase).
All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.