Thrombopoietin (TPO) Neutralizing Antibody, Mouse Monoclonal [MA211N-100 or MA211N-025] 

 

Nplate (Romiplostim) is a thrombopoietin receptor (TPO-R) agonist marketed by Amgen* for the treatment of thrombocytopenia (Reference 1, see below). It has been approved for the treatment of both adult and pediatric patients with immune thrombocytopenia (ITP), a condition that occurs when the blood platelet count is too low. Nplate (Romiplostim) is a recombinant human IgG Fc fusion protein produced in E. coli (2).

GenHunter has 3 neutralizing monoclonal antibodies against Nplate (Romiplostim) [Clone 5D5-G2, Clone 2A3-B6, & 5E7-F8], 2 neutralizing antibodies against Thrombopoietin (TPO), a TPO fusion protein, & Ba/F3 (c-Mpl) cells (see below). The Nplate NAbs have specificity against TPO-R Mimetic Peptide (Nplate) and can be used by CRO or biotech companies for GCP testing of clinical samples in biosimilar development. Nplate is a fusion protein analog of thrombopoietin (TPO), a hormone that regulates platelet production.

Nplate (Romiplostim) Neutralizing Antibody Determination: Nplate neutralizing antibody assay for the three mAbs was carried out with Ba/F3 (c-Mpl) cell bioassay method in which Ba/F3 (c-Mpl) cells were grown in the presence of Nplate with or without the mAbs. EC50 (see data in specifications tab below) was determined at the concentration where a mAb caused 50% inhibition of the cell proliferation.

Nplate (Romiplostim) & TPO Antibody/Antigen Products Available:

Monoclonal Antibody Description	Clone	Isotype	Specificity	Volume	Applications	Cat. No.	Click to Order
Nplate (Romiplostim) Neutralizing Antibody, Mouse Monoclonal (Clone 5D5-G2)	5D5-G2	IgG1, Kappa	TPO-R Mimetic Peptide (Nplate)	100µg or 25µg	NAb,ELISA,WB	MA201N-100 MA201N-025	Order
Nplate (Romiplostim) Neutralizing Antibody, Mouse Monoclonal (Clone 2A3-B6)	2A3-B6	IgG1, Kappa	TPO-R Mimetic Peptide (Nplate)	100µg or 25µg	NAb,ELISA,WB	MA202N-100 MA202N-025	Order
Nplate (Romiplostim) Neutralizing Antibody, Mouse Monoclonal (Clone 5E7-F8)	5E7-F8	Not tested	TPO-R Mimetic Peptide (Nplate)	100µg or 25µg	NAb,ELISA,WB	MA203N-100 MA203N-025	Order
Thrombopoietin (TPO) Neutralizing Antibody, Mouse Monoclonal	38C9-G5	Not tested	Thrombopoietin (TPO)	100µg or 25µg	NAb,ELISA,WB	MA211N-100 MA211N-025	Order
Thrombopoietin (TPO) Neutralizing Antibody, Rabbit Polyclonal	N/A	Not tested	Thrombopoietin (TPO)	100µg or 25µg	NAb,ELISA,WB	MA215N-100 MA215N-025	Order
Thrombopoietin (TPO), C-terminal His-tagged Fusion Protein				100µg or 25µg		MP401-100 MP401-025	Order
Ba/F3 (c-Mpl) Cells					Nplate neutralizing bioassay	MA221	Order

We also have many other primary antibodies, secondary antibodies, & antigen products (CLICK HERE or the names below) for:  SARS-CoV-2 (COVID-19), Human IL-24, Human IL-20R2, Nplate (Romiplostim), TPO (Thrombopoietin), Human VEGF-R, Collagen, Endo180 (uPARAP, CD280), Tumor Necrosis Factor (TNF) superfamily, SIRPα (Signal-regulatory protein alpha), Rabies virus, TRIMER-TAG, RSV (Respiratory Syncytial Virus), HSV-2 (Herpes Simplex Virus 2), VZV (Varicella Zoster Virus), Influenza (Flu-A & Flu-B), PIV (Parainfluenza virus), hMPV (human metapneumovirus), AP (alkaline phosphatase), PEDV (porcine epidemic diarrhea virus), PRRSV (porcine reproductive respiratory sydrome virus), plus Secondary Antibodies (for human, porcine, feline, & canine), & Vaccine Adjuvents.

References:

1. Kuter, D.J. et al: Efficacy of romiplostim in patients with chronic immune thrombocytopenic purpura: a double-blind randomised controlled trial. (2008) The Lancet. 371:395-403. https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(08)60203-2/abstract
2. Cines, D.B. et al: Romiplostim. (2008) Nature Reviews Drug Discovery. 7:887-888. https://www.nature.com/articles/nrd2741

*Note: Nplate^® & Amgen^® are registered trademarks of Amgen Inc. None of our antibodies/antigens are manufactured by, affiliated with, or endorsed by Amgen. They are included for comparative purposes only.

All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.