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GenHunter DNA Sequencing Service

GenHunter DNA Sequencing Service imagesGenHunter is a leading service provider of Sanger DNA sequencing for many universities & biotech companies in the Nashville area due to our high-quality sequences, fast turnaround time, friendly customer service, & free pickups (available at Vanderbilt locations). Ask about our special offers to try us today!

How to Order

Click on the order form here & email it to sequencing@genhunter.com

  • Deadlines: Submit the order form by 10am CST M-F to qualify for next-day results (usually ready the next morning, 1-3 business days max)
  • Pickups
    • Vanderbilt pickups are from 10:30am-noon M-F
    • Samples ready by 10:30am may qualify for next-day results
    • At your lab, create a designated pickup location with a GenHunter-labeled box or rack in a -20C, 4C, or cold room for our courier to find, placing tubes in the same order as they occur on the form

Submission Guidelines

Click on this sequencing information sheet for submission guidelines & more

  • Tubes: samples & primers in separate tubes
    • 1.5 mL flip-top tubes
    • 8-strip PCR tubes - may be used for exact multiples of 8 (no partial strips or empty tubes), corresponding with adjacent lines on the form
  • Volume: 8 µL per reaction (10 µL if sample concentration is low)
  • Concentration: can be outside the recommended ranges below, but dilute if more than 50% above
    • PCR Products: 20-40 ng/µL
    • Plasmids: 100-300 ng/µL
    • BACs & gDNA: 500-1000 ng/µL
  • Sample Prep: use a high-quality commercial purification kit such as Qiagen
    • Elute DNA in autoclaved dH2O (not buffer)
    • For genomic DNA, please isolate the DNA and then perform PCR amplification of the specific target sequence prior to submission
      • Bacterial gDNA may be submitted for direct sequencing without PCR amplification
  • Primer Prep
    • Primers should be an exact match with the template, ~17-25 bp long, with an annealing site more than 30 bp away from the target
    • To convert ng/µL to µM: µM = [(ng/µL)/(Molecular Weight)] x 1000   {Molecular Weight ≈ (# bases in primer) x 32}

Saving Tubes

Tubes are saved for 2 weeks by default

  • Samples & primers submitted during this time frame may be requested for use in additional reactions - simply submit an order form and any tubes that are not already in our possession
  • Primers that are frequently used by your lab may be requested to be saved for a longer period so you do not have to resubmit them each time!

Results

Results are usually ready the following morning & are guaranteed within 1-3 business days max

Troubleshooting

Below is a quick guide to help prevent some common issues such as noisy data, failed reactions, & short reads - if you have any questions before or after sequencing, please let us know & we will be happy to help!

  • Run samples on an agarose gel to check the concentration & quality of DNA
  • Prep samples with high-quality kits such as Qiagen - use fresh reagents & elute with autoclaved dH2O instead of buffer
  • Check samples for the presence of multiple priming sites or templates, which can cause mixed/overlapping reads
  • Confirm that the primer is an exact match with the template
  • Use primers that have not been degraded from too many freeze-thaw cycles or long-term storage at 4C

Advantages

  • High Quality
    • Long reads up to 1050 bp on our ABI 3130xl, with good resolution over 700-800 bp
    • Polite & knowledgeable tech support & customer service
  • Fast Turnaround 
    • Most results are usually ready the next morning
  • Convenient
    • Fast response time
    • Complimentary pickups at all Vanderbilt labs & drop-offs accepted from locations everywhere
    • Located off of I-65 in Nashville, we are in driving distance of many area universities & biotech companies
  • Trusted Source
    • Hundreds of customers including Vanderbilt labs have used our service for over a decade
  • Free Repeats
    • Repeats are run as soon as space is available, with one free repeat per reaction as needed

Pricing

  • Standard Sanger Sequencing 
    • 1-15 reactions: $8.15 / rxn
    • 16+ reactions:  $8 / rxn
    • Print-out of chromatogram: add $0.25 / rxn
  • Bulk Sequencing (96-Well Plate)
    • Use this order form for plates
    • $7 / rxn ($672 / plate) for one primer w/ 96 templates or one template w/ 96 primers
  • Run Only (you do the reactions & we run it)
    • 1-15 reactions: $6 / run
    • 16+ reactions:  $4 / run

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Since 2003, GenHunter has been working with researchers at many local institutions including Vanderbilt University, Tennessee State University (TSU), Middle Tennessee State University (MTSU), Meharry Medical College, and Lipscomb University--we are proud to provide quality data to help facilitate the important research of our biomedical community!

 

Questions or comments? Contact our sequencing department using the form below or email sequencing@genhunter.com.

 


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