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GenHunter DNA Sequencing Service

GenHunter DNA Sequencing Service imagesGenHunter is a leading service provider of Sanger DNA sequencing for many universities & biotech companies in the Nashville area due to our high-quality sequences, fast turnaround time, friendly customer service, & free pickups (available at Vanderbilt locations). Ask about our special offers to try us today!

How to Order

Click on the order form here & email it to

  • Deadlines: Submit the order form by 10am CST M-F to qualify for next-day results (usually ready the next morning, 1-3 business days max)
  • Pickups
    • Vanderbilt pickups are from 10:30am-noon M-F
    • Samples ready by 10:30am may qualify for next-day results
    • At your lab, create a designated pickup location with a GenHunter-labeled box or rack in a -20C, 4C, or cold room for our courier to find, placing tubes in the same order as they occur on the form

Submission Guidelines

Click on this sequencing information sheet for submission guidelines & more

  • Tubes: samples & primers in separate tubes
    • 1.5 mL flip-top tubes
    • 8-strip PCR tubes - may be used for exact multiples of 8 (no partial strips or empty tubes), corresponding with adjacent lines on the form
  • Volume: 8 µL per reaction (10 µL if sample concentration is low)
  • Concentration: can be outside the recommended ranges below, but dilute if more than 50% above
    • PCR Products: 20-40 ng/µL
    • Plasmids: 100-300 ng/µL
    • BACs & gDNA: 500-1000 ng/µL
  • Sample Prep: use a high-quality commercial purification kit such as Qiagen
    • Elute DNA in autoclaved dH2O (not buffer)
    • For genomic DNA, please isolate the DNA and then perform PCR amplification of the specific target sequence prior to submission
      • Bacterial gDNA may be submitted for direct sequencing without PCR amplification
  • Primer Prep
    • Primers should be an exact match with the template, ~17-25 bp long, with an annealing site more than 30 bp away from the target
    • To convert ng/µL to µM: µM = [(ng/µL)/(Molecular Weight)] x 1000   {Molecular Weight ≈ (# bases in primer) x 32}

Saving Tubes

Tubes are saved for 2 weeks by default

  • Samples & primers submitted during this time frame may be requested for use in additional reactions - simply submit an order form and any tubes that are not already in our possession
  • Primers that are frequently used by your lab may be requested to be saved for a longer period so you do not have to resubmit them each time!


Results are usually ready the following morning & are guaranteed within 1-3 business days max


Below is a quick guide to help prevent some common issues such as noisy data, failed reactions, & short reads - if you have any questions before or after sequencing, please let us know & we will be happy to help!

  • Run samples on an agarose gel to check the concentration & quality of DNA
  • Prep samples with high-quality kits such as Qiagen - use fresh reagents & elute with autoclaved dH2O instead of buffer
  • Check samples for the presence of multiple priming sites or templates, which can cause mixed/overlapping reads
  • Confirm that the primer is an exact match with the template
  • Use primers that have not been degraded from too many freeze-thaw cycles or long-term storage at 4C


  • High Quality
    • Long reads up to 1050 bp on our ABI 3130xl, with good resolution over 700-800 bp
    • Polite & knowledgeable tech support & customer service
  • Fast Turnaround 
    • Most results are usually ready the next morning
  • Convenient
    • Fast response time
    • Complimentary pickups at all Vanderbilt labs & drop-offs accepted from locations everywhere
    • Located off of I-65 in Nashville, we are in driving distance of many area universities & biotech companies
  • Trusted Source
    • Hundreds of customers including Vanderbilt labs have used our service for over a decade
  • Free Repeats
    • Repeats are run as soon as space is available, with one free repeat per reaction as needed


  • Standard Sanger Sequencing 
    • 1-15 reactions: $8.15 / rxn
    • 16+ reactions:  $8 / rxn
    • Print-out of chromatogram: add $0.25 / rxn
  • Bulk Sequencing (96-Well Plate)
    • Use this order form for plates
    • $7 / rxn ($672 / plate) for one primer w/ 96 templates or one template w/ 96 primers
  • Run Only (you do the reactions & we run it)
    • 1-15 reactions: $6 / run
    • 16+ reactions:  $4 / run


Since 2003, GenHunter has been working with researchers at many local institutions including Vanderbilt University, Tennessee State University (TSU), Middle Tennessee State University (MTSU), Meharry Medical College, and Lipscomb University--we are proud to provide quality data to help facilitate the important research of our biomedical community!