For immunoblotting of AP fusion proteins

This kit contains the AP Antibody (rabbit polyclonal) which is specific to human placental secreted AP as well as two controls for AP Western blots. The antibody works optimally when used for Western blot analysis of secreted AP fusion proteins from culture media. It should be noted that although this antibody works extremely well for Western blot detection of AP fusion proteins, AP itself (with a MW of 67 KD) may not be detected directly from the culture media due to the amount of albumin which runs at a similar MW. Therefore, the purified AP from human placenta and a known soluble receptor AP fusion protein are provided as positive controls for the antibody. In addition, this antibody only recognizes the denatured form of AP. The Monoclonal AP Antibody (Cat. # Q320) can be used for applications where recognition of the native form is required.

Detection Limit: 20 mU of AP

The kit is shipped on dry ice via overnight delivery.

Detailed protocol included.