- Western Blotting
- The PerfectMembrane
Note: All PerfectWestern Containers have quantity discounts when you order 5, 10, 20, or 50 containers (or sets) which are automatically applied. Discounts are:
20% off (for 5-9 containers)
30% off (for 10-19 containers)
35% off (for 20-49 containers)
40% off (50+ containers) the standard 1-4 container price.
The PerfectMembrane precisely fits inside our Medium, Mini Square, or Extra Large PerfectWestern containers with room for efficient movement. Our membrane offers convenience at an excellent price - about half the cost of other pre-cut membranes!
Still cutting your own Western blot membranes? Save time, effort, and reduce waste with The PerfectMembrane!
Available in standard 0.45 um and 0.2 um filter pore sizes
Move well during incubation
Save time and materials
Half the cost of competitors
Available in 10, 25, and 50 sheet packs
8.3 x 6 cm (Medium, for standard mini-gels such as Bio-Rad Mini-PROTEAN 3, etc)
8.3 x 7.3 cm (Mini Square, for longer mini-gels such as Invitrogen/Novex XCell SureLock Mini, etc)
13.5 x 8.5 cm (Extra Large, for larger precast gels such as Bio-Rad Criterion or Invitrogen Midi)
PVDF, 0.45 um: Standard PVDF with 0.45 um pore size for most proteins (> 20 kDA)
PVDF, 0.2 um: 0.2 um pore size for smaller proteins (< 20 kDA) or Sequencing
PVDF, Fluor: Optimized for fluorescence, extremely low background compatible with most imagers, 0.45 um pore size)
Nitrocellulose: Standard NC with 0.45 um pore size for most proteins (> 20 kDA)
|Membrane Type||Cat. No.*||Pore Size||Insulin binding||BSA binding|
|PVDF 0.45 µm||B301-50||0.45 µm||~160 µg/cm^2||~215 µg/cm^2|
|PVDF 0.2 µm||B302-50||0.2 µm||~262 µg/cm^2||~340 µg/cm^2|
|PVDF Fluor||B303-50||0.45 µm||~155 µg/cm^2||~205 µg/cm^2|
|Nitrocellulose||B311-50||0.45 µm||~80 µg/cm^2||~100 µg/cm^2|
*Cat. No. is for the Medium size with 50 membranes. For other sizes or membrane quantities, please click on the Size, Membrane Material, and Quantity above. It will then display the price and the Cat. No. (SKU).
Should I use PVDF or Nitrocellulose?
GenHunter prefers PVDF for the reasons shown below. However, we know that some customers prefer Nitrocellulose, so we offer both types.
Advantages of PVDF:
- Better protein retention (Pluskal, BioTechniques. 1986, 4:272)
- Much more durable allowing easier handling due to higher mechanical strength
- Higher binding capacity (100-200ug/cm^2) vs. NC (80-100ug/cm^2)
- Lower concentrations detected: Requires 5X less antigen, 20X less serum, 10X less secondary antibody (Dalessio, J. Clin. Micro. 1992, 30:1005)
- Superior chemical resistance makes it ideal for staining and re-probing
Please click on the Size, Membrane Material, and Quantity above. It will then display the price and the Cat. No. (SKU).