For Cell Staining

The AP Assay Reagent S is formulated specifically for cell staining or Western blotting analysis of enzymatic activity of the alkaline phosphatase (AP). The AP substrate BCIP upon dephosphorylation forms an insoluble blue precipitate, thus it can be used for tissue or cell staining for the presence of receptors to which the ligand-AP fusion proteins bind. This assay kit can also be used during expression cloning of a receptor (Cheng and Flanagan, 1994, Cell 79:157-168).

Availabe in 10 mL or 100 mL sizes

Detailed protocol included.

Reference:

Flanagan, J. G. and Leder, P. (1990). The kit Ligand: A cell surface molecule altered in steel mutant fibroblasts. Cell 63, 185-194.