GH2/P3 Supercompetent Cells
For transformation of pAPtag-2 and pAPtag-4 vectors
The pAPtag-2 and pAPtag-4 AP-fusion cloning vectors (AP-TAG Kit A) contain the supF gene which confers both ampicillin and tetracycline resistance when transformed into the GH2/P3 Supercompetent Cells. pAPtag-2 and pAPtag-4 vectors will not confer antibiotic resistance in an E. coli host which does not contain the P3 episome. A tube of 1000X AT antibiotics mix is included for your convenience to prepare ampicillin (25 mg/mL) and tetracycline (10 mg/mL) plates for 1 L of LB-agar.
Detailed protocol included.
| GH2/P3 Supercompetent Cells |
|GH2/P3 Supercompetent Cells||5 x 0.4 mL|
1000X AT Antibiotics Mix
(Amp and Tet)
2 mLs (5 x 0.4 mL tubes)
Also includes 1 mL of 1000X AT Antibiotics Mix (Amp & Tet)
GH2/P3 Supercompetent Cells - Cat. No. T601