For transformation of pAPtag-2 and pAPtag-4 vectors

 

The pAPtag-2 and pAPtag-4 AP-fusion cloning vectors (AP-TAG Kit A) contain the supF gene which confers both ampicillin and tetracycline resistance when transformed into the GH2/P3 Supercompetent Cells. pAPtag-2 and pAPtag-4 vectors will not confer antibiotic resistance in an E. coli host which does not contain the P3 episome. A tube of 1000X AT antibiotics mix is included for your convenience to prepare ampicillin (25 mg/mL) and tetracycline (10 mg/mL) plates for 1 L of LB-agar.

 

Detailed protocol included.

GH2/P3 Supercompetent Cells
GH2/P3 Supercompetent Cells	5 x 0.4 mL
1000X AT Antibiotics Mix      (Amp and Tet)	1 mL