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RNAimage DD Kits (for standard Differential Display)
Product Description
For mRNA Differential Display with three one-base anchored oligo-dT primers and rationally designed arbitrary 13mers.
These kits feature the latest generation of isotopic mRNA Differential Display technology (U.S. Patents 5,262,311; 5,599,672; 5,665,547; 5,965,409; Japanese Patent 2843675 and pending patents). Each kit contains all the reagents needed for differential display except Taq DNA polymerase and alpha-[33P] dATP. The MessageClean® Kit is highly recommended to remove trace amounts of DNA contamination from RNA samples prior to DD.
| RNAimage Kit 1 | |
| 1. 5X Reverse Transcription Buffer | 200 µL |
| 2. MMLV Reverse Transcriptase (100 u/µL) | 40 µL |
| 3. dNTP (250 µM) | 200 µL |
| 4. H-T11G Anchor Primer | 300 µL |
| 5. H-T11A Anchor Primer | 300 µL |
| 6. H-T11C Anchor Primer | 300 µL |
| 7. 10X PCR Buffer | 500 µL |
| 8. dNTP (25 µM) | 500 µL |
| 9. H-AP1 Arbitrary Primer | 150 µL |
| 10. H-AP2 Arbitrary Primer | 150 µL |
| 11. H-AP3 Arbitrary Primer | 150 µL |
| 12. H-AP4 Arbitrary Primer | 150 µL |
| 13. H-AP5 Arbitrary Primer | 150 µL |
| 14. H-AP6 Arbitrary Primer | 150 µL |
| 15. H-AP7 Arbitrary Primer | 150 µL |
| 16. H-AP8 Arbitrary Primer | 150 µL |
| 17. Control RNA (1 µg/µL) | 1 µL |
| 18. Glycogen (10 mg/mL) | 200 µL |
| 19. dH2O | 1.2 mL |
| 20. Loading Dye | 500 µL |
Each kit contains all the reagents needed for differential display except RNA samples, Taq DNA polymerase, and isotope. The MessageClean® Kit is also highly recommended prior to differential display to remove trace amounts of DNA contamination from RNA samples.
The major improvements over the original RNAmap™ technology are: (1) Optimized primer length ensures excellent reproducibility and maximized coverage of expressed genes. (2) One-base anchored primers minimize the redundancy of the displayed cDNA and simplifies the reverse transcription reactions. (3) Attached Hind III site to the primers facilitates the manipulation of the cDNA after cloning.
We also offer the RNAspectra FDD (Fluorescent Differential Display) Kits.
All kits are shipped on dry ice via overnight delivery. A detailed step-by step protocol is included.
RNAimage® Kits 2-10 contain all the components of Kit 1, but each has 8 different arbitrary 13mers (H-AP9 to H-AP80).
One could start with any RNAimage® Kit, since the sequences are random and designed to be maximally different from one another, but to ensure a maximum coverage, multiple kits may be needed.
| RNAimage Kit 1 | |
| 1. 5X Reverse Transcription Buffer | 200 µL |
| 2. MMLV Reverse Transcriptase (100 u/µL) | 40 µL |
| 3. dNTP (250 µM) | 200 µL |
| 4. H-T11G Anchor Primer | 300 µL |
| 5. H-T11A Anchor Primer | 300 µL |
| 6. H-T11C Anchor Primer | 300 µL |
| 7. 10X PCR Buffer | 500 µL |
| 8. dNTP (25 µM) | 500 µL |
| 9. H-AP1 Arbitrary Primer | 150 µL |
| 10. H-AP2 Arbitrary Primer | 150 µL |
| 11. H-AP3 Arbitrary Primer | 150 µL |
| 12. H-AP4 Arbitrary Primer | 150 µL |
| 13. H-AP5 Arbitrary Primer | 150 µL |
| 14. H-AP6 Arbitrary Primer | 150 µL |
| 15. H-AP7 Arbitrary Primer | 150 µL |
| 16. H-AP8 Arbitrary Primer | 150 µL |
| 17. Control RNA (1 µg/µL) | 1 µL |
| 18. Glycogen (10 mg/mL) | 200 µL |
| 19. dH2O | 1.2 mL |
| 20. Loading Dye | 500 µL |
Each kit contains all the reagents needed for differential display except RNA samples, Taq DNA polymerase, and isotope. The MessageClean® is also highly recommended prior to differential display to remove trace amounts of DNA contamination from RNA samples.
Documents
| | Cat. No. |
| RNAimage Kit 1 | G501 |
| RNAimage Kit 2 | G502 |
| RNAimage Kit 3 | G503 |
| RNAimage Kit 4 | G504 |
| RNAimage Kit 5 | G505 |
| RNAimage Kit 6 | G506 |
| RNAimage Kit 7 | G507 |
| RNAimage Kit 8 | G508 |
| RNAimage Kit 9 | G509 |
| RNAimage Kit 10 | G510 |
We also offer the RNAspectra FDD (Fluorescent Differential Display) Kits.
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