For complete cleaning of DNA contamination from RNA.
This very popular kit contains everything required for the complete removal of trace amounts of chromosomal DNA contamination from RNA isolated by any method, including GIT-CsCl gradient centrifugation and the simple one-step acid phenol extraction. This step is absolutely essential for a successful mRNA Differential Display. GenHunter's GH-DNase I is designed especially for the complete digestion of single- and double-stranded DNA with absolute integrity of the RNA before differential display. DNase I from other vendors are mostly used for DNase footprinting, thus may be contaminated with RNase.
| MessageClean Kit |
|1. 10X Reaction Buffer||140 µL|
|2. GH DNase I (RNase free, 10 u/µL)||20 µL|
|3. 3M NaOAc||140 µL|
|4. H20 (DEPC treated)||1 mL|
|5. RNA Loading Mix||400 µL|
The figure above shows the result of our stringent QC of the MessageClean® kit. l-DNA or total RNA isolated with GenHunter's RNApureTM reagent were incubated without (-) or with (+) GH-DNase I for 30 min and then analyzed by gel analysis. Note only the l-DNA, not the RNA, was degraded .
MessageClean Kit (for 20 RNA sample cleanings) - Cat. No. M601