In Differential Display Methods and Protocols, Drs. Peng Liang and Arthur B. Pardee have for the first time assembled a comprehensive
review of the state of the art of their powerful new methodology and its practical applications. The book's pioneering contributors describe all the
major elements of this novel technology, including optimal primer designs, DD using fluorescence detection, cloning family-specific genes and major
causes and solutions for false positives. Also provided are numerous examples, along with detailed experimental procedures, in which differentially
expressed genes have been successfully identified in diverse biological systems ranging from plants to songbirds to humans.
Comprehensive and on the cutting-edge, Differential Display Methods and Protocols provides readers with precise new tools for studying exactly how
gene expression is regulated throughout the development of a living organism, and how the failure of this intricate control mechanism leads to
pathological complications. This novel and productive methodology, fully detailed here is already playing a major role in the development of selective
antagonists and inhibitors for treating cancer, cardiovascular disease, CNS disorders, and inflammation and tissue repair. Now scientists everywhere
can successfully apply this powerful set of techniques using the step-by-step procedures of its innovators.
306pp. 1997, Humana Press
Edited by Peng Liang and Arthur B. Pardee
The first book dedicated to differential display technology.
Contains numerous step-by-step protocols and improvements by experts in the field.
Discusses major avoidable causes for false positives.
Includes applications from a wide range of biological systems from plants to animal brains.
Offers suggestions for the future development of the technology.
Treats all aspects of differential display for identifying and cloning differentially expressed genes.
Presents numerous examples of successfully identified differentially expressed genes.
Truly a road map for all those who are interested in differential display technology.
|Differential Display Methods and Protocols||HP1
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This week-long intensive lab course focuses on all aspects of the most up-to-date differential display techniques in identifying and
cloning differentially expressed genes. The workshop is offered by Vanderbilt University each year in June or July. The organizer of the
workshop is Dr. Peng Liang. In four workshops since 1997, there have been 93 students from 17 countries. Class size is typically 20 to 30.
Companies including GenHunter, Qiagen, NEN-Dupont, Eppendorf, National Diagnostics and Sarstedt co-sponsor the workshops. Areas to be
- Principle of Differential Display technique
- Removal of chromosomal DNA from RNA
- Differential Display using one-base anchored primers
- Reamplifying and cloning cDNAs of interest
- Northern blot for confirmation of differential gene expression
For more detailed information regarding registration and availability of space for the workshop,
please fill out the Information Request form right here on
our web site.