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GH2/P3 Supercompetent

For transformation of pAPtag-2 and pAPtag-4 vectors

The pAPtag-2 and pAPtag-4 AP-fusion cloning vectors contain the supF gene which confers both ampicillin and tetracycline resistance when transformed into the GH2/P3 Supercompetent Cells. pAPtag-2 and pAPtag-4 vectors will not confer antibiotic resistance in an E. coli host which does not contain the P3 episome. A tube of 1000X AT antibiotics mix is included for your convenience to prepare ampicillin (25 mg/mL) and tetracycline (10 mg/mL) plates for 1 L of LB-agar.

Detailed protocol included.


 Cat. No.
GH2/P3 Supercompetent Cells
T601


AT Antibiotics Mix

For selection of pAPtag-2 and pAPtag-4 plasmids.

Each tube of AT antibiotics mix is conveniently packaged to prepare ampicillin (25 mg/mL) and tetracycline (10 mg/mL) plates for 1 L of LB-agar.

 Cat. No.Volume
AT Antibiotics Mix (1000X)
Q601
1 mL


GH Competent Cells

For transformation of pAPtag-5 vector

The pAPtag-5 AP fusion cloning vector contains the ampicillin resistance gene. It can be easily and efficiently transformed and propagated in GH Competent cells.

 Cat. No.Size
GH Competent Cells
L301
6 x 0.5 mL




 
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