
For transformation of pAPtag-2 and pAPtag-4 vectors
The pAPtag-2 and pAPtag-4 AP-fusion cloning vectors contain the supF gene which confers both ampicillin and tetracycline resistance when
transformed into the GH2/P3 Supercompetent Cells. pAPtag-2 and pAPtag-4 vectors will not confer antibiotic resistance in an E. coli host which does
not contain the P3 episome. A tube of 1000X AT antibiotics mix is included for your convenience to prepare ampicillin (25 mg/mL) and tetracycline
(10 mg/mL) plates for 1 L of LB-agar.
Detailed protocol included.
| | Cat. No. |
| GH2/P3 Supercompetent Cells | T601 |
|

For selection of pAPtag-2 and pAPtag-4 plasmids.
Each tube of AT antibiotics mix is conveniently packaged to prepare ampicillin (25 mg/mL)
and tetracycline (10 mg/mL) plates for 1 L of LB-agar.
| | Cat. No. | Volume |
| AT Antibiotics Mix (1000X) | Q601 | 1 mL |
|

For transformation of pAPtag-5 vector
The pAPtag-5 AP fusion cloning vector contains the ampicillin resistance gene. It can be
easily and efficiently transformed and propagated in GH Competent cells.
| | Cat. No. | Size |
| GH Competent Cells | L301 | 6 x 0.5 mL |
|
|