For AP Activity Assay

The AP assay reagent A is formulated specifically for measuring the enzymatic activity of the alkaline phosphatase (AP). The secretion of human placenta AP or a secreted ligand(receptor)-AP fusion protein can be easily monitored by this assay using the culture media in which the transfected cells are grown. The dephosphorylation of the p-Nitrophenyl Phosphate by AP leads to the generation of yellow color which serves as both qualitative (by eye) and quantitative (Absorbance 405 nm) measurement of AP activity.

10 mL

Detailed protocol included.

Reference:

Flanagan, J. G. and Leder, P. (1990). The kit Ligand: A cell surface molecule altered in steel mutant fibroblasts. Cell 63, 185-194.