Mycoplasma contamination is a major problem for cell culture in biomedical and biopharmaceutical research, with rates at 15-30%. There is no method for visual confirmation of mycoplasma and contamination can have disastrous effects on key characteristics and functions of cells, thus compromising any research done with them. Cipro (ciprofloxacin hydrochloride) is the standard antibiotic for rapid and efficient decontamination.
GenHunter offers 99.5% pure Cipro in either powder form or a ready-to-use 1000X solution.
Lowest Prices Guaranteed.
Applications:
- Treatment of mycoplasma-infected cell lines
- Decontamination of visible Pen-Strep resistant
bacteria
- Treatment of newly acquired cell lines as a
precaution
- Use during all transfections to prevent
contamination from plasmid
Purity: 99.5% pure
Stability:
Powder: 2 years at 4 degrees C
Ready-to-Use Solution: 1 year at 4 degrees C.
Product information:
Ciprofloxacin Hydrochloride (Powder)
1 g $79.50 Cat. No. Q901-1G Click here to order
5 g $325.50 Cat. No. Q901-5G Click here to order
Larger packaging size available upon request
Ciprofloxacin Hydrochloride (Ready-to-Use Sterile Solution)
Concentration: 10 mg/mL (1000X)
1 mL (can treat 1 L of media) $24.95 Cat. No. Q902-1ML Click here to order
10 x 1 mL (can treat 10 L of media) $149.50 Cat. No. Q902-10ML Click here to order
50 mL (can treat 50 L of media) $395 Cat. No. Q902-50ML Click here to order
Larger packaging size available upon request
FOR RESEARCH USE ONLY
MSDS available upon request
Background information:
Mycoplasmas are very small prokaryotes that do not have a rigid cell wall and this elasticity allows them to easily change shape and pass through filters. Contamination of cell culture media by these organisms has become a recurring problem for both biomedical research laboratories and biopharmaceutical industry. Depending on the information source, the contamination rate of cell cultures in any given laboratory varies between 15 and 30%.
Mycoplasmas are covert contaminants. The absence of any visible signs of mycoplasma contamination, such as cytopathogenicity and changes in turbidity or pH (even in heavily contaminates cultures), leads to a false sense of security. As a result, some mycoplasma-contaminated cultures have unknowingly been used in research for years. However, although they may be unseen, mycoplasmas still have serious effects on the key characteristics and functions of the cells and compromise any research done with them.
Fortunately, at the end of the last century, German pharmaceutical giant Bayer introduced the first broad-spectrum fluoroquinolone class of drug, Ciprofloxacin (Cipro), which is extremely effective for the treatment of 14 types of microbial infections, including Mycoplasmas. Shortly after, it was reported that a two-week treatment of Cipro at 10 ug/mL was able to eradicate 7 different strains of mycoplasma from contaminated cell lines, without any noticeable side effects to the cultured cells (see reference below). Since then, Cipro has been the standard antibiotic for rapid and efficient de-contaminations of mycoplasma infected cell lines in cell culture. It has become a good practice for many leading research laboratories to routinely treat any newly acquired cell line with Cipro to ensure the cells are free from any potential mycoplasma contamination that could spread to other cell lines being used in the lab.
Although Cipro has been a popular drug in the clinic, Bayer does not market it for cell culture applications. To this end, GenHunter Corporation is proud to make Cipro readily available at the most affordable price to tens of thousands of research laboratories.
Reference:
Schmitt, K. et al: A safe and efficient method for elimination of cell culture mycoplasmas using ciprofloxacin. Journal of Immunology Methods. 1988. 109:17-25.
Click here to read original manuscript
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