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DD technology featured in new PNAS paper and other Nature journals
Killin, a new p53 tumor-suppressor gene target, discovered by FDD

Killin inhibits DNA replication
Dr. Peng Liang's laboratory at Vanderbilt has identified a long sought-after p53 tumor-suppressor target gene, which they dubbed "Killin" for its ability to cause cell death by arresting DNA replication. Dr. Liang is the co-inventor of DD and his laboratory worked with GenHunter to perform a saturation Fluorescent Differential Display (FDD) screen of p53 target genes and identified Killin as a unique mediator of p53 in S-phase check point control and apopotosis.

To read this April 2008 PNAS publication, click here.

Legend for figure at right:
RFP-Killin inhibits DNA replication in vivo. The RFP-Killin in-frame fusion protein (Upper) or RFP control (Lower) expression vectors were transiently transfected into Cos-E5 cells Twenty-four hours after transfection, S phase cells undergoing DNA replication were visualized after 30-min pulse label with BrdU followed by FITC-labeled anti-BrdU antibody staining (in green). For cells in which the RFP-Killin (red) and BrdU signals (green) colocalized, the bulk of DNA replication foci (origins of replication) were missing in the area where the RFP-Killin foci reside. The overlay of fluorescent signals from BrdU labeling with RFP-Killin (Merge) always exhibit a mutually exclusive pattern, in contrast to control cells transfected with RFP alone. DAPI was used to stain DNA (nuclei).

Nature Methods publications:
Recognizing the tremendous and continuing impact of DD technology on biomedical research in the past 15 years, Nature Methods featured the DD methodology as a Classic Protocol. The accompanying commentary by Michael Eisenstein traced back to the days when Drs. Arthur Pardee and Peng Liang invented the ingenious method. The two inventors also offered their perspectives on DD versus DNA microarrays.

To read the "Classic Protocol", Click here.
Complete reference:
Differential display polymerase chain reaction. Nature Methods. 2006, 3:325-326.

To read Eisenstein's commentary, Click here.
Complete reference:
Eisenstein, M.: A look back: putting differences aside. Nature Methods. 2006, 3:324.

Nature Protocols publication:
With continued interest and demand in DD technology for the most accurate analysis of differential gene expression, GenHunter has compiled a step-by-step protocol for its state-of-the-art DD technology in both fluorescent digital and radioactive formats. This most up-to-date and authoritative DD methodology cited more than any other competitive methodology including DNA microarrays, has now just been published by Nature Protocols. Without a doubt, the protocol provides the most accurate roadmap to successful hunting for truly differential expressed genes in any eukaryotic species.

To read the protocol, Click here.
Complete reference:
Liang, P., Meade, J., and Pardee, A.B.: A protocol for differential display of mRNA expression using either fluorescent or radioactive labeling. Nature Protocols. 2007, Mar 15, 2:457-470.

Differential Display Methods and Protocols, 2nd edition book:
Get the newest book dedicated to the most popular differential gene expression technology.

For more information on the "Differential Display Methods and Protocols, 2nd edition" book including the Table of Contents and Editorial Reviews click here.

Carrying on the high standards and building on the huge success of the first edition of Differential Display Methods and Protocols, Peng Liang et al. recently released a second edition of the book. This edition includes a varying collection of 20 chapters highlighting both recent Differential Display (DD) methodological refinements (Chapters 1-8) and some of the most elegant examples of DD research applications (Chapters 9-20).

These well-versed authors explain and highlight all the latest DD refinements, including a new mathematical model, automation of fluorescent DD and digital data acquisition/analysis, capillary electrophoresis, a prototype computer program for automatic band identification, DD screening linking a band directly to a given gene, prokaryotic DD, and a technique combining Microarrays and DD.

The collection includes outstanding examples of applications of DD in the discovery of important genes involved in cancer, viral infection, prion disease, ovulation, circadian clock, floral color, transcription repression gene silencing, mRNA polymorphism, and protein-RNA interactions.

As with all "Methods in Molecular Biology" books, each author includes specific hints/notes to ensure success.

State-of-the-art and highly practical, the book offers gene hunters the possibility of genome-wide comprehensive screening, as well as a proven road map for successful gene expression profiling.

Humana Press, 2005, Hardcover, 325 Pages
Cat. No: HP2, Price: $99

Get the First Edition book ($99 value) for only $25 when you purchase the Second Edition. Total cost for both books = $124. Click here to order both books - Offer #1 (Quote HP2a)

2) Get 10% off the Second Edition. Click here to order the new book at 10% off - Offer #2 (Quote HP2b)

For more information on the "Differential Display Methods and Protocols, 2nd edition" book including the Table of Contents and Editorial Reviews click here.

Because of several major advantages, Differential Display (DD) continues to lead other competing technologies including Microarray, Oligo Array, and SAGE in total publication numbers. To see the latest comparison of publications numbers as well as a list of advantages of DD technology, please click here.

GenHunter Corporation, in business since 1992, is the world leader in DD technology, offering both DD Kits and Fluorescent DD (FDD) Services. Our FDD Service has been utilized by both academic and industry customers including 52 different institutions from 8 different countries. For a list of our FDD Service clients, click here.

GenHunter has also complied a list of more than 500 selected DD publications in at least 113 different journals, organized chronologically and by fields of application, showing the wide range of organisms and experiments that can be performed.

Please feel free to contact us with any questions or comments.

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